miR-877-5P acts as a tumor suppressor in multiple myeloma by targeting MAPK8: a study on prognostic value and functional mechanism.

چکیده اصلی

OBJECTIVES: This study aimed to investigate Investigate the expression level, prognostic value, and biological function of miR-877-5p in MM. METHODS: A total of 103 MM patients and 98 non-tumor controls were included in the study. miR-877-5p expression level was verified by RT-qPCR, and its prognostic value was evaluated through Kaplan-Meier and the Cox regression model. MM cell proliferation and apoptosis were assessed with the CCK‑8 assay and flow cytometry. The potential target genes of miR-877-5p were predicted by bioinformatics analysis and verified by dual luciferase assay. Rescue assays confirmed that MAPK8 mediates the regulatory function of miR-877-5p. RESULTS: miR-877-5p was reduced in MM, and its low level was associated with a poorer overall survival of patients, being an independent prognosticator of poor survival in MM. Function experiments confirmed that overexpression of miR-877-5p could inhibit the proliferation of MM cells and promote apoptosis. Mechanistically, MAPK8 was a direct downstream target of miR-877-5p. MAPK8 was upregulated in MM and negatively correlated with miR-877-5p levels. Rescue assays were conducted to verify that MAPK8 overexpression reversed the effect of miR-877-5p on MM cell proliferation and apoptosis. DISCUSSION: These findings indicate that miR-877-5p exerts tumor-suppressive functions in MM, likely through negative regulation of MAPK8. The miR-877-5p/MAPK8 axis may represent a novel regulatory pathway involved in MM progression. CONCLUSIONS: miR-877-5p acts as a tumor suppressor in MM and may serve as a potential biomarker for prognosis assessment. Its regulatory role in MM progression is likely mediated through targeting and inhibiting MAPK8.

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