Physicochemical, structural, and in-vitro release properties of low methoxyl pectin-based particle gel beads incorporating apigenin-loaded porous starch for colon-targeted delivery system.

چکیده اصلی

The objective of this study was to evaluate the physicochemical, structural, and in vitro release properties of low methoxyl pectin (LMP)-based particle gel beads incorporating apigenin-loaded porous starch (APS) as a colon-targeted delivery system. LMP-based particle gel beads incorporating APS were prepared as a colon-targeted delivery system for apigenin (AP). Porous starch (PS) was prepared using α-amylase and amyloglucosidase for 12 h of enzymatic treatment. AP was then adsorbed onto PS to prepare APS. APS was then incorporated into an additional LMP-based hydrogel bead layer with different concentrations of CaCl2 (2%, 3%, and 4%) to fabricate particle gel beads. XRD analysis revealed that APS was successfully incorporated into LMP-based particle gel beads. According to FT-IR analysis, AP was adsorbed onto PS by hydrogen bonding, and LMP-based particle gel beads were prepared via ionic crosslinking between LMP and CaCl2. FE-SEM images revealed that the surface of the particle gel beads became smoother, and their internal structure was more compact as concentrations of CaCl2 increased. The encapsulation efficiency of the particle gel beads increased from 87.95% to 93.54%, with an increase in CaCl2 concentration from 2% to 4%, respectively. In vitro release study indicated that LMP-based particle gel beads maintained better stability than APS throughout the simulated oral, gastric, and intestinal conditions, effectively delivering AP to the colon. Within simulated colon conditions, increased CaCl2 concentration delayed AP release, demonstrating enhanced controlled delivery. Results from the storage stability evaluation additionally indicated that LMP-based particle gel beads provided higher stability for apigenin than APS.

متن کامل اصلی