A multi-functional microneedle patch loaded with mupirocin and L-arginine nanoparticles for enhancing antibacterial activity, anti-inflammation and diabetic wound healing.

چکیده اصلی

Chronic wounds pose notable clinical challenges due to persistent infection, inflammation, and impaired tissue regeneration. We designed a multi-functional microneedle patch (MR-NPs-MNs) comprising chitosan-based microneedles encapsulating PLGA nanoparticles co-loaded with mupirocin and L-arginine. As a key component, L-arginine significantly enhances anti-inflammatory effects by reducing pro-inflammatory cytokines and effectively promotes tissue repair by regulating oxidative stress and facilitating angiogenesis. The PLGA nanoparticles MR-NPs exhibited stable physical properties (181.7 ± 5.875 nm particle size, 22.4 ± 0.529 mV zeta potential) and were incorporated into microneedles capable of withstanding 40.38 N compression. Notably, MR-NPs-MNs outperformed conventional mupirocin ointment, achieving a 99.82 ± 0.15% antibacterial rate (vs. 90.69 ± 1.11% for the ointment), superior biofilm inhibition (96.82 ± 0.83% and 77.8 ± 5.32%), and enhanced transdermal drug delivery. In vitro, MR-NPs-MNs increased NO production, reduced TNF-α, IL-6 and ROS levels, and promoted wound healing. In diabetic wound models, MR-NPs-MNs increased the healing rate by 1.54-fold, significantly reduced bacterial counts (P < 0.01) by day 9, and improved key healing parameters including collagen matrix reorganization, robust neovascularization, and functional tissue formation. With its biocompatible components, this system provides a potential local therapeutic platform for infected diabetic wound treatment.

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